Table 1 Plasmids used in this study.
From: Arginine glycosylation regulates UDP-GlcNAc biosynthesis in Salmonella enterica
Construct | Plasmid | Source |
|---|---|---|
Recombinant proteins | ||
Flag-SseK1 | pFLAG-CTC-sseK1 | |
Flag-SseK1 (HEN) | pFLAG-CTC-sseK1 H244A E255A N256A | |
GST-SseK1 | pET42a-sseK1 | |
GST-SseK1 (HEN) | pET42a-sseK1 H244A E255A N256A | |
His-NagC | *pTac-nagC | This study |
His-GlmS | pTac-glmS | This study |
His-GlmM | pTac-glmM | This study |
His-GlmU | pTac-glmU | This study |
His-GlmR | pTac-glmR | This study |
His-GNA1 | pTac- Saccharomyces cerevisiae GNA1 | This study |
His-NagC R25A | pTac-nagC R25A | This study |
His-NagC R35A | pTac-nagC R35A | This study |
His-NagC R54A | pTac-nagC R54A | This study |
His-NagC R59A | pTac-nagC R59A | This study |
His-GlmR R110A | pTac-glmR R110A | This study |
His-GlmR R212A | pTac-glmR R212A | This study |
His-NagC quadruple R/K | pTac-nagC R25K R35K R54K R59K | This study |
Transcriptional fusions | ||
nagB::gfp | pHG156a- nagB::gfp | This study |
glmU::gfp | pHG156a- glmU::gfp | This study |
glmR::gfp | pHG156a- glmR::gfp | This study |
