Figure 4
From: Multimodal bioimaging using nanodiamond and gold hybrid nanoparticles
Visualization of ND@Au interaction with A549 cell via Raman mapping (I) and mapping of fluorescence (II). (I): (a) The cell is shown via mapping the distribution of intensity at 2800–3100 cm−1; (b) ND@Au NP are shown using ND Raman signal (mapping of the signal in range 1300–1350 cm−1); (c) merging of (a) and (b); (d) the optical image; (e) Raman spectra according to (c) measured in points marked 1 (cell) and 2 (the cell with ND@Au). (f) 3D Raman mapping reconstructed via the spectral signal intensity. (II) (a) The cell is shown via mapping the distribution of intensity at 565–575 nm; (b) ND@Au NP are shown via fluorescence of ND SiV center (730–750 nm); (c) merging of (a) and (b); (d) the optical image; (e) spectra measured from (c) point 1 (cell) and point 2 (the cell with ND@Au); (f) 3D mapping re-constructed via spectra intensity. The concentration of ND@Au was 20 µg/ml. excitation 488 nm.
