Figure 5
From: Multimodal bioimaging using nanodiamond and gold hybrid nanoparticles
2D and 3D Raman mapping of A549 cell after incubation for 4 h with various concentrations of ND@Au: (a) 10 µg/ml, (b) 20 µg/ml and (c) 50 µg/ml. For cell imaging Raman intensity spatial distribution in the range 2800–3100 cm−1 is mapped (shown in red); ND@Au is shown via ND Raman signal in the 1300–1350 cm−1 range (green). (d) the reconstructed image of a Raman mapping from Principle Component Analysis (PCA) with K-means cluster analysis, shown the relative positions of each component, nucleus (blue), ND@Au (green), cytoplasm (red).
