Figure 2

Photomicrographs for GAL (A–D,F), CD11b (E,F) and DAPI (F) in the cerebellar tentorium by a conventional fluorescence microscope (A–C) and a confocal laser scanning microscope. Panels (D–F) are from the same field of views. GAL-immunoreactivity is expressed by round (an arrowhead) and oblong cells (a double arrowhead), and varicose nerve fibers (arrows) in the thick dura mater (A). Gray arrows indicate the direction toward the bone (A). Panels (C) and (D) show round and oblong types, respectively, of GAL-ir cells with some processes (arrowheads). GAL- and CD11b-immunoreactivity are co-expressed by round (arrowheads) and oblong (double arrowheads) cells (D–F). Bars = 50 µm (A), 20 µm (D) and 10 µm (B,C). Panels (D–F) are at the same magnification.