Figure 4

Photomicrographs for GAL (A–D) and DAPI (B,D) in the dura mater by a conventional fluorescence microscope. Bar graphs indicating the density of GAL-ir nerve fibers in the dura mater. Perivascular nerve fibers with varicosities (arrows in A,B) contain GAL-immunoreactivity. GAL-ir nerve fibers (arrows in C,D) are also detected in the vicinity of oblong (arrowheads in C,D) GAL-ir cells. BV blood vessel. Bar = 50 µm (A) and 20 µm (C). Panels (A,B) and (C,D) are at the same magnifications, respectively. The density of GAL-ir nerve fibers in the cerebral falx and cerebellar dura mater is significantly higher than in other portions of the cerebral dura mater (Tukey’s test, **p < 0.01; *p < 0.05). The data were obtained from three sections of the cerebral falx, 12 sections of other portions of the cerebral dura mater, and two sections of the cerebellar dura mater in each of the four animals.