Figure 5

Photomicrographs for GAL (A,D,G,J), PGP9.5 (B), CGRP (E,H,K), and merged and DAPI (C,F,I,L) in the dura mater by a conventional fluorescence (A–F) and a confocal laser scanning (G–L) microscope. Panels (A–C), (D–F), (G–I), and (J–L) are from the same fields of view, respectively. Panels (J–L) were obtained from 13 sections, respectively, at 0.5 µm interval. Isolated (A–C) and perivascular (D–F) nerve fibers, and nerve fibers apposed to cells (G–L) with GAL- (A,D,G,J), PGP9.5- (B,C), and CGRP-immunoreactivity (E,F,H,I,K,L) show similar distributions. Arrowheads in (G–I) and (J–L) indicate round and oblong types of GAL-ir cells, respectively. Bars = 50 µm (A,D) and 10 µm (G,J). Panels (A–C), (D–F), (G–I), and (J–L) are at the same magnifications, respectively.