Figure 6

Photomicrographs for GAL (A,B,C,E), CGRP (A,D,E), ED1 (B,E) and DAPI (A,B) in the dura mater by a confocal laser scanning microscope. Panels (C–E) are from the same field of view. CGRP-ir varicose fibers (an arrows in A) in the vicinity of a GAL-ir cell (an arrowhead in A) often lack GAL-immunoreactivity. A GAL-ir cell (a double arrowhead in B) is also detected in the vicinity of ED1-ir cells without GAL-immunoreactivity (an arrowheads in B). A nerve fiber that co-expresses GAL- and CGRP-immunoreactivity (arrows in C–E) are observed in the vicinity of an ED1-ir cell (arrowheads in C–E). Bars = 10 µm (A–C). Panels (C–E) are at the same magnification.