Figure 7

Photo for FG in the brain (A), schematic drawings of TG (B,C), and photomicrographs for FG (D), GAL (E), and CGRP (F) in the TG by a conventional fluorescence microscope (A,D–F). FG fluorescence is detected in the dorsal portion of the cerebellar tentorium (arrow in A). Squares in (B) and (C) show the areas of panels (C) and (D–F), respectively. FG-positive neurons are mainly located in the ophthalmic region of the TG (B,C). These neurons contain GAL- and/or CGRP-immunoreactivity (C). Panels (D–F) are from the same field of view. A TG neuron retrogradely labeled with FG from the cerebellar dura mater (arrow in D) contains both GAL- (arrow in E) and CGRP-immunoreactivity (arrow in F). Bars = 5 mm (A) and 20 µm (D). Panels (D–F) are at the same magnification. Bar graphs showing the cell size spectrum of FG-positive TG neurons with and without GAL- and CGRP-immunoreactivity. Data are obtained from 262 FG-positive TG neurons in four animals.