Figure 4 | Scientific Reports

Figure 4

From: NU-9 improves health of hSOD1G93A mouse upper motor neurons in vitro, especially in combination with riluzole or edaravone

Figure 4

Sholl analysis of UMNs from WT-UeGFP and hSOD1G93A-UeGFP mice with or without various drug treatments. (a) Representative images of UMNs in dissociated cell cultures of motor cortex isolated from WT-UeGFP, hSOD1G93A-UeGFP mice treated with SFM (b), with 400 nM of NU-9 (c), with 500 nM riluzole, (d), 1 µM edaravone (e), combination of NU-9 and riluzole (f), combination of NU-9 and edaravone (g) for 3 days in vitro. (h) Sholl analyses of WT-UeGFP UMN treated with SFM (blue), hSOD1G93A-UeGFP UMN treated with SFM (red), and NU-9 (green). (i) Sholl analyses of hSOD1G93A-UeGFP UMN treated with NU-9 (green), edaravone (orange), riluzole (purple), and SFM (red). (j) Sholl analyses of hSOD1G93A-UeGFP UMN treated with NU-9 and edaravone (dark blue), NU-9 and riluzole (brown), NU-9 alone (green), and SFM (red). 0–200 µm radius range is enlarged in the inset for clarification. Scale bar = 20 µm, n = 3 biological replicates. SFM = serum free medium; G = WT-UeGFP; GS = hSOD1G93A-UeGFP.

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