Figure 4
(a) Intravital microscopic imaging of mouse brain pial venules demonstrating changes in blood–brain barrier/microvascular permeability. Pial venules of 50-75 µm diameter were visualized at 40 × magnification. The imaging was started 10 min after injury or drug administration. Images and video were recorded every 20 min for comparison. Dotted lines have been added to better differentiate the pial venules. (b) Graphical plotting of BBB Permeability as ΔI. Two-way ANOVA showed no statistically significant difference among the groups at the initial time point of 10 min after injury. Increased microvascular hyperpermeability at 30, 50, 70 min of TBI was observed in comparison to sham (*p < 0.05; n = 5). TBI + Doxycycline group showed a significant decrease in hyperpermeability compared to TBI group (*p < 0.05; n = 5). No difference in Sham versus TBI + Doxycycline groups observed.
