Figure 1
From: A suspension technique for efficient large-scale cancer organoid culturing and perturbation screens
Alternative organoid culturing techniques and longitudinal study to assess 5% ECM culturing feasibility. (a) Representative images of H&E staining and IHC of panCK (pan-cytokeratin), Ki67 and P53 in two organoids cultured in either 80% ECM domes or 5% ECM suspension. All images are 10X magnification. (b) Study design comparing the 5% ECM with standard conditions. Samples were taken at the start (T0), after approximately 1–3 (T1) and 6 (T2) months, and then used for genomic and phenotypic analyses. (c) Representative images of three organoid models over the first 35 days of the study. (d) Images of three organoid models growing in T75 flasks in 5% ECM. Organoid images are 10X magnification.
