Table 1 Three methodologies tested for organoid culturing compared to standard organoid culturing protocols.
From: A suspension technique for efficient large-scale cancer organoid culturing and perturbation screens
Technique | Organoid growth | Scalability | Time (benefit) | Cost (Benefit) |
|---|---|---|---|---|
Standard conditions 80% ECM | Yes | + | + | + |
5% ECM | Yes | +++ | +++ | +++ |
Microcarriers | Yes | − | + | + |
Spinner flasks | No |
