Figure 3

Effects of olanzapine and AuNCs co-treatment on H1R-AMPK signaling, POMC protein expression and POMC immunofluorescence staining in the hypothalamus. (a) TEM image of a hypothalamic slice at 6th h after IP injection of 20 mg/kg AuNCs in rats. The presence of AuNCs was marked by red arrows. (b) Representative western blot figures of H1R, AMPK, pAMPK and POMC in the hypothalamus after co-treatment of olanzapine and AuNCs. (c–e) Densitometry analysis of H1R expression (c), pAMPK/AMPK (d) and POMC expression (e). (f–k) POMC immunofluorescence staining in the hypothalamic Arc of rats in CON (f), OLZ (g), O + AuNCs H (h), O + AuNCs L (i), AuNCs H (j) group and the corresponding quantification of POMC fluorescence intensity (k). n = 4/group. All data were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.0001, OLZ vs. CON; ^#p < 0.05, ^##p < 0.01, ^###p < 0.0001, OLZ + AuNCs H vs. OLZ; ^$p < 0.05, OLZ + AuNCs L vs. OLZ. Original figures were shown in Fig. S8.