Figure 4

Effects of olanzapine and AuNCs co-treatment on the protein expression of BAT thermogenic markers. (a) Representative western blot figures of UCP-1, PGC-1α, PPAR-α, PPAR-γ protein expression in BAT. (b–e) Densitometry analysis of the protein expression of UCP-1 (b), PGC-1α (c), PPAR-α (d) and PPAR-γ (e). (f) Immunohistochemistry of UCP-1 and PGC-1α and H&E staining of BAT. (g–i) Quantification of relative positive area for UCP-1 (g), PGC-1α (h) and multilocular brown adipocytes (i). n = 4/group. (j) Concentrations of AuNCs in BAT of rats after administration of saline or 20 mg/kg AuNCs for 2 h. n = 3/group. All data were presented as mean ± SEM. *p < 0.05, ***p < 0.0001, OLZ vs. CON; ^#p < 0.05, ^##p < 0.01, ^###p < 0.0001, OLZ + AuNCs H vs. OLZ; ^$p < 0.05, OLZ + AuNCs L vs. OLZ. Original figures were shown in Fig. S9.