Table 1 The dissociation constants given as mean ± SEM of the selected foldamers and the S100ome measured by direct fluorescence polarization.
From: Promiscuity mapping of the S100 protein family using a high-throughput holdup assay
Kd (μM) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
fIF | fRF | fTM | fTW | fWW | fTI | fIL | fRR | fVL | fWL | fYF | |
S100A1 | 210 ± 13 | 420 ± 81 | > 1000 | 330 ± 20 | 110 ± 19 | > 1000 | 160 ± 24 | 280 ± 38 | 97 ± 12 | 53 ± 5.2 | 110 ± 9.8 |
S100A2 | 38 ± 1.5 | 340 ± 21 | > 1000 | 170 ± 5.5 | 65 ± 1.2 | > 1000 | 78 ± 8.7 | 61 ± 5.5 | 210 ± 110 | 11 ± 0.39 | 26 ± 2.1 |
S100A3 | 180 ± 8.4 | > 1000 | > 1000 | > 1000 | 310 ± 21 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100A4 | 93 ± 2.4 | 460 ± 23 | > 1000 | 180 ± 46 | 160 ± 9.8 | > 1000 | 150 ± 4.8 | > 1000 | 210 ± 8.7 | 46 ± 1.6 | 110 ± 5.2 |
S100A5 | 27 ± 0.53 | 46 ± 1.2 | 200 ± 9.2 | 62 ± 1.4 | 77 ± 2.8 | 270 ± 14 | 75 ± 3.6 | 45 ± 0.81 | 110 ± 5.4 | 14 ± 0.55 | 32 ± 1.0 |
S100A6 | 57 ± 1.8 | 290 ± 14 | 520 ± 57 | 160 ± 8.1 | 100 ± 3.2 | 700 ± 63 | 82 ± 5.4 | > 1000 | 170 ± 13 | 30 ± 1.7 | 42 ± 3.3 |
S100A7 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | 23 ± 1.5 | > 1000 |
S100A8 | 750 ± 46 | > 1000 | > 1000 | 770 ± 180 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | 74 ± 3.5 | 180 ± 42 |
S100A9 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100A10 | 97 ± 2.5 | 320 ± 19 | > 1000 | 170 ± 5.6 | 150 ± 5.2 | > 1000 | 72 ± 1.6 | > 1000 | 110 ± 5.5 | 23 ± 0.7 | 70 ± 3.5 |
S100A11 | 280 ± 15 | 1000 ± 220 | > 1000 | 570 ± 68 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100A12 | 590 ± 39 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | 250 ± 110 | > 1000 |
S100A13 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100A14 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100A15 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | 39 ± 2.4 | > 1000 |
S100A16 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | 300 ± 130 | > 1000 |
S100B | 140 ± 7.9 | 230 ± 5.9 | > 1000 | 280 ± 17 | > 1000 | > 1000 | 63 ± 4.4 | 210 ± 26 | 50 ± 6.4 | 11 ± 0.81 | 28 ± 1.7 |
S100G | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
S100P | 140 ± 5.8 | 190 ± 17 | > 1000 | 170 ± 9.8 | 190 ± 6.9 | > 1000 | 330 ± 92 | > 1000 | > 1000 | 75 ± 5.8 | 79 ± 5.7 |
S100Z | 480 ± 31 | 890 ± 120 | > 1000 | 520 ± 59 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 | > 1000 |
