Figure 6 | Scientific Reports

Figure 6

From: Rhoifolin from Plumula Nelumbinis exhibits anti-cancer effects in pancreatic cancer via AKT/JNK signaling pathways

Figure 6

Rhoifolin suppressed proliferation, migration and invasion, and accelerated apoptosis by JNK and AKT pathways in pancreatic cancer cells. PANC-1 and ASPC-1 cells were dealt with rhoifolin, SC79, or/and SP600125, respectively. (A) Cell proliferation analysis of PANC-1 and ASPC-1 cells after processing. (B) Cell apoptosis was tested with TUNEL staining. (C) The rate of TUNEL positive cells was calculated. (D) Flow cytometry was utilized to monitor cell apoptosis. (E,F) The migrated and invaded cells were assessed with Transwell. (G) Western blotting analysis of AKT, p-AKT, JNK, p-JNK and apoptosis-related proteins in the processed cells. (H) Relative quantitative analysis of the western blotting results.

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