Figure 1 | Scientific Reports

Figure 1

From: A mouse model with widespread expression of the C9orf72-linked glycine–arginine dipeptide displays non-lethal ALS/FTD-like phenotypes

Figure 1

GR50 neurotoxic profile in vitro. Primary cortical neurons (A) and motor neurons (B) co-transfected with 125 ng td-tomato and 400 ng of GFP-tagged GR constructs of length 25, 50, 100, or GFP. Scale bar 100 µm. Inset shows cells that are double-positive for td-tomato and GFP-tagged construct of interest. Closed arrows indicate cells that survive over the 14-day observation period. Open arrows indicate cells that die over the time-course of the experiment. Log-rank longitudinal survival assessment of co-transfected td-tomato and GFP-GR25, 50, 100, or GFP alone quantified for primary corticals in (C) and motor neurons (D) over 14 days. Cells (m) pooled from n = 3 independent experiments. Primary cortical neurons m = 319 (GFP), 302 (GR25), 348 (GR50), and 263 (GR100). ****p < 0.0001. Primary motor neurons m = 149 (GFP), 105 (GR25), 163 (GR50), and 169 (GR100). ***p = 0.0001, ****p < 0.0001. Cell counts, p-values, and hazard ratios summarized in Tables 1 and 2.

Back to article page