Figure 6

Motor and behavioral phenotypes in GR50 expressing mice. (A) Weight of female (left) and male (right) control-GFP and GR50-GFP mice at 3, 6, 9, and 12 months. Females: control-GFP n = 15, 15, 13, 6; GR50-GFP n = 14, 11, 11, 6. Males: control-GFP n = 12, 13, 11, 5; GR50-GFP n = 14, 11, 11, 6. Mean ± s.e.m. Females: 3-months p = 0.9945, 6-months p = 0.9934, 9-months p = 0.9184, 12-months p = 0.9373. Males: 3-months p = 0.9997, 6-months p > 0.9999, 9-months p = 0.9976, 12-months p = 0.0473. (B) Rotarod performance of female (left) and male (right) control-GFP and GR50-GFP mice at 3, 6, 9, and 12 months. Females: control-GFP n = 14, 9, 9, 4; GR50-GFP n = 14, 11, 11, 6. Males: control-GFP n = 12, 13, 10, 5; GR50-GFP n = 10, 9, 8, 3. Mean ± s.e.m. Females: 3-months p = 0.8103, 6-months p = 0.9430, 9-months p = 0.0686, 12-months p = 0.0177. Males: 3-months p = 0.1998, 6-months p = 0.8804, 9-months p = 0.3002, 12-months p = 0.0117. (C) Hindlimb grip strength measurements of female (left) and male (right) control-GFP and GR50-GFP mice at 3, 6, and 9 months. Females: n = 5 per genotype; Males: n = 7 control-GFP, 5 GR50-GFP. Mean ± s.e.m. Females: time x genotype p = 0.4314. Males: time x genotype p = 0.5490. (D) Male total distance traveled in Open Field Assessment. Control-GFP n = 7, 7, 4; GR50-GFP n = 5, 4, 5. Mean ± s.e.m. 3-months p = 0.9654, 6-months p = 0.4424, 9-months p = 0.9810. (E) Male average speed in Open Field Assessment. Control-GFP n = 7, 7, 4; GR50-GFP n = 5, 5, 5. Mean ± s.e.m. 3-months p = 0.9535, 6-months p = 0.2208, 9-months p = 0.9759. (F) Male time spent in the center zone in Open Field Assessment. Control-GFP n = 7, 7, 4; GR50-GFP n = 5, 4, 5. Mean ± s.e.m. 3-months p > 0.9999, 6-months p = 0.7245, 9-months p = 0.9974. (G) Representative track plots of male control-GFP and GR50-GFP mice at 3-months. (H) Female total distance traveled in Open Field Assessment. Control-GFP n = 5, 5, 5; GR50-GFP n = 5, 5, 4. Mean ± s.e.m. 3-months p = 0.1890, 6-months p = 0.5160, 9-months p = 0.0158. (I) Female average speed in Open Field Assessment. Control-GFP n = 5, 5, 5; GR50-GFP n = 5, 5, 4. Mean ± s.e.m. 3-months p = 0.4798, 6-months p = 0.6563, 9-months p = 0.1392. (J) Female time spent in the center zone in Open Field Assessment. Control-GFP n = 5, 5, 5; GR50-GFP n = 5, 5, 4. Mean ± s.e.m. 3-months p = 0.0056, 6-months p = 0.8140, 9-months p = 0.4907. (K) Representative track plots of female control-GFP and GR50-GFP mice at 3-months. For all datasets, Two-way ANOVA with Sidak’s multiple comparisons test was used for statistical analysis.