Table 3 Safety of DC vaccines loaded with COVID-19 peptides.

From: First computational design using lambda-superstrings and in vivo validation of SARS-CoV-2 vaccine

Conditiona

Cell viabilityb

Apoptosisc

NT

99 ± 0.3%

3 ± 0.3%

CoVPSA

98 ± 0.2%

4 ± 0.2%

DC-peptide CONT

97 ± 0.5%

4.5 ± 0.4%

  1. aDC cells were incubated with 50 µg/mL of peptides for 16 hours.
  2. bCell viability was explored after trypan blue staining and microscopy was used to count viable (non-stained) and non-viable (blue stained) cells. Results are expressed as the percentage of viable versus total cells (viable and non-viable cells).
  3. cApoptosis is detected after DC staining with the DNA marker 7-AAD-PE and the apoptotic marker Annexin-V-APC. Results show the percentage of apoptotic cells ± SD of triplicate samples. All experiments were performed at least three times.
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