Figure 2

MALDI MS imaging of lipids in neuronal and non-neuronal compartments of D. rerio. (a,e) H&E (hematoxylin and eosin) staining of the sagittal cryosections in adult zebrafish showing detailed structures within the gills’ filaments, eye (retinae, lens), brain (telencephalon, tectum opticum, cerebellum) and liver. (b) RGB Overlay of PC (O-32:0) [M+H]⁺ (red) and PC (30:0) [M+K]⁺ (green). (c,d), (g–i) Positive ion mode MS images. (c) Ion image of PC (O-32:0) [M+H]⁺ showing high intensity in the region of the gills’ filaments (white arrow). (d) Ion image of PC (30:0) [M+K]⁺ visualizing the lens and the retina of the eye (white arrow). (f) RGB Overlay of PC (O-32:0) [M+H]⁺ (red colored), PC (O-36:1) [M+K]⁺ (blue colored) and PC (40:6) [M+H]⁺ (green colored). (g) Ion image of PC (O-32:0) [M+H]⁺ shows distribution with high intensity in non-neuronal tissue, exemplarily shown for muscle tissue (white arrow). (h) Ion image of PC (O-36:1) [M+K]⁺, illustrates the structural differentiation within the tectum opticum and parts of the cerebellum. (i) Ion image of PC (40:6) [M+H]⁺ shows distribution within the brain and eye and presents the liver as relative homogenous tissue (white arrows).