Figure 2
From: Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster
Effect of X-ray irradiation on the homozygous Otu11 (w+; + ;CG4603Δ101/2/CG4603Δ101/2) mutant and the heterozygous Usp51 mutant (w+; + ;Usp51/TM6b GFP) Drosophila. (a) and (b) L3 DUB mutant larvae were irradiated with 40 Gy X-ray, and the rates of pupation and eclosion were measured (a and b, respectively). (c) The percentage of wing and eye abnormalities was represented. In both the mutants and the control lines, the number of Drosophila with abnormale eye and wing phenotype was compared to the number of the hatched Drosophila which survived the X-ray treatment. (d) The detected wing and eye defects in adult DUB mutants are depicted. For the statistical analysis, one-way ANOVA in combination with Tukey’s post-hoc test was used. Significant values are indicated as follows: *P < 0.05, ***P < 0.001, ****P < 0.0001. Data were obtained from three independent experiments.
