Figure 1
From: Heterogeneous fates of simultaneously-born neurons in the cortical ventricular zone
Isochronic early AP-born neurons have diverse laminar fates. (a) Schematic representation of the FlashTag (FT) labeling strategy. Arrowheads, FT pulse injection in the embryonic lateral ventricle. Black line, continuous BrdU delivery by osmotic pump. P7: collection day. (b) Left: Photomicrographs illustrating the results of FT pulse and chronic BrdU labeling (further details in Fig. S1). Circled: FT+BrdU- (i.e. directly AP-born) neurons, which are studied here. Right: P7 laminar distribution of AP-born neurons at different embryonic ages. Horizontal bar: median. n = 3 pups/condition (p1, p2, p3). Scale bar: 200 µm. (c) Unsupervised hierarchical clustering of centered radial distribution of all experiments. Right: Mean-normalized interquartile range (Two-Way ANOVA on radial position standard deviation, by Early vs. Late (E11.5, E12.5, E13.5 vs. E14.5, E15.5, E16.5) time: P = 7.89e-6. Two sample Welch test Early vs. Late ****P = 0. 0,001,063). (d) Schematic summary of the findings. DL Deep layers; E Embryonic day; L Layer; P Postnatal day; p1, p2, p3 Pup number; S1 Primary somatosensory cortex; SL Superficial layers; SP Subplate.
