Figure 1 | Scientific Reports

Figure 1

From: scTEM-seq: Single-cell analysis of transposable element methylation to link global epigenetic heterogeneity with transcriptional programs

Figure 1

scTEM-seq accurately measures DNA methylation at TE sites. (A) Schematic representation of combined scTEM-seq and scRNA-seq workflow. (B) Unique SINE Alu sites measured in KG1a cells compared to raw sequencing reads. (C) DNA methylation levels as measured by scTEM-seq in KG1a cells with and without DAC treatment. Coloured lines show average DNA methylation levels at SINE Alu sites for each treatment group measured in bulk samples. DAC treated KG1a cells show a heterogeneous loss of DNA methylation. (D) Unique SINE Alu sites measured in AML01 patient blasts compared to raw sequencing reads. (E) DNA methylation levels in untreated AML01 patient blasts measured by scTEM-seq.

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