Figure 2
PACE4 isoforms immunohistochemical analysis of PCa specimens. (A) Representative images of prostate epithelial cells stained for either PACE4-altCT or PACE4-FL with the different scoring units used. Magnification: 20 ×, scale bars represent 50 µm. A 40 × magnification figure is also found in Supplementary Fig. 1 to encompass cellular localization of immunohistochemical signal. (B) Averaged immunoscores for PACE4-altCT on the TMA cores of normal (n = 92), PIN (n = 67), Gleason 3 (n = 85), 4 (n = 49) and 5 (n = 12). Gleason scores are the primary Gleason pattern for each patients. Data are means ± SEM, all individual data are shown as dots. (C) Averaged immunoscores for PACE4-FL on the TMA cores of normal (n = 88), PIN (n = 64), Gleason 3 (n = 79), 4 (n = 47) and 5 (n = 8). Differences between the number of patients analyzed for this figure compared to PACE4-altCT (Fig. 2B) are due to core detachment and/or histological differences on successive slides. (D) Plots of immunoscoring for PACE4-altCT and PACE4-FL immunoscores for each tumor scored. The Spearman correlation r is calculated from the entire data set without regrouping scores together in intervals, histogram are means ± SEM. Statistical analysis is based on One-way ANOVA.
