Figure 4 | Scientific Reports

Figure 4

From: PACE4-altCT isoform of proprotein convertase PACE4 as tissue and plasmatic biomarker for prostate cancer

Figure 4

Development, optimization and validation of PACE4 sandwich ELISA assays. Antibody validation assays for (A) PACE4 capture antibody, (B) total-PACE4 sandwich (2nd) antibody and (C) PACE4-altCT sandwich (2nd) antibody. (D) Representative standard curves of total PACE4 and (E) PACE4-altCT. Assays from A-E use recombinant PACE4 (diluted in human serum diluted 1;5 with PBS). (F) Quantitation of PACE4 concentration in the conditioned culture media of control (shNon-Target) and PACE4-knockdown (shPACE4) DU145 and LNCaP PCa cells (n = 3). * indicates P value < 0.05. Data are means ± SEM.

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