Figure 4
From: DNA damage alters EGFR signaling and reprograms cellular response via Mre-11
DNA damage alters gene expression profile and cellular response to HB-EGF triggering. (A) HF were treated as in Fig. 1A. After 30 and 90 min RNA was extracted and was subjected to RNAseq analysis. Differentially expressed genes altered with higher than twofold changes were selected. A list of IPA canonical pathways that are enriched in bleomycin treated cells stimulated with HB-EGF compared with those from HB-EGF stimulated mock cells (upper left panel). IPA upstream functional analysis was used to predict the top upstream regulators from differentially expressed genes (lower left panel). Gene set enrichment analysis (GSEA) pathways that are up-regulated (red) or down-regulated (blue) in response to HB-EGF treatment in bleomycin as compared with mock-treated cells (right panel). (B) Mock and peroxide (500 µM) treated cells were pulsed with BrdU for two hours immediately after treatment. After 24 h the cells were fixed and analyzed by flow cytometry. The percentage of BrdU positive cells is shown. Representative results of three independent experiments are shown. (C) Cells were treated as above and after 12 h were collected and stained with Annexin-V. The percentage of Annexin-V positive cells of three independent experiments was normalized to mock (set as 1). *p < 0.05; **p < 0.005; ns: non-significant.
