Figure 3
From: Identification of lamprey variable lymphocyte receptors that target the brain vasculature
YSD screening of the BBBVLR library. (A) Representative brightfield images of yeast binding to confluent MBEC monolayers at the end of each biopanning round. Yeast are the small round cells and examples are indicated by black arrowheads. (B) Percentage of applied yeast that were recovered by binding to ECM and MBEC during each round of biopanning. (C) After the third round of biopanning, MBEC-binding VLR clones were identified via a 96-well biopanning assay using yeast displaying RBC36 VLR as a non-binding VLR control. Scale bars = 50 μm.
