Figure 3

Deletion analysis of hnRNPD promoter. (A) Schematic diagram for generation of various deletions constructs of human hnRNPD promoter. (B) A series of promoter fragment having a common 3ʹ end (+ 257) but 5ʹ lacking increasing number of nucleotides (− 1088, − 824, − 605, − 255 and − 126) were PCR amplified as described in Materials and Methods and cloned separately upstream to luciferase reporter gene in pGL3-Basic plasmid to generate deletion constructs pVKS-2, pVKS-3, pVKS-4, pVKS-5 and pVKS-6 respectively. The individual constructs were co-transfected with pRL-TK plasmid in SCC-4 cells and the luciferase activities were measured 48 h post-transfection. Other details are same as described in Fig. 1. Values are mean ± SD from four independent experiments performed in triplicate. The results were statistically analyzed using a paired two tailed Student’s t-test and values significantly different from pVKS-1 have been marked by stars (**P ≤ 0.01, *P ≤ 0.05).