Figure 3
SCAPE imaging of pollen grains showed detailed substructure within each grain. (a,b) Three-dimensional images of two different pollen grains show different details. The pollen in (a) showed small spikes on the surface, while the pollen in (b) showed the smooth surface of a multi-grain conglomeration. (c) Projections along Cartesian planes were color matched to the planes in (a). Each projection showed the sharp, micrometer sized spikes on the surface of the pollen. Color profiles along sharp features are plotted on top of the individual projections. (d) Projections along Cartesian planes are color matched to the planes in (b). Each projection showed the internal boundaries between the multiple grains. Scale bars and axes all represent 10 µm. The excitation power was 1.0 mW after the excitation objective, the distance between planes was 0.25 µm, and the frame acquisition time was 30 ms.
