Figure 7
From: Integrated analysis of expression profiles with meat quality traits in cattle
Validation of miRNA endogenous targets via dual-luciferase reporter and Western blot assay. (a) Showed the activity of PLIN5 and bta-miR-409a by binding assay. (b) Presented the validating of PLIN5 as a target of bta-miR-209a at the protein level using western blot. Three tracks were captured from the original full-length membrane image and were presented in the same order consistent with (a). (c) is nucleotide sequences of binding sites located in the 3′ UTR of PLIN5. The sample derives from the same experiment, and that blots were processed in parallel. The party of the full-length membrane image is presented in Supplementary Fig. S8.
