Figure 2

KIF4A overexpression is associated with fewer tumor-infiltrating CD8⁺ T lymphocytes. (A,B) Representative images of immunohistochemical staining of CD8⁺ in bladder cancer tissues (4 × 100, A: low expression, B: high expression). (C) CD8⁺ TILs frequencies in BC samples from BC with high KIF4A expression were compared to those from BC with low KIF4A expression (2-tailed Student’s t test, **P < 0.01). (D) CD8⁺ TILs frequencies were correlated with KIF4A expression in BC tissues. The data was normalized to the GAPDH control. Lines indicate linear regression. R Spearman’s rank correlation coefficients, P the corresponding P values. TIL tumor-infiltrating lymphocytes.