Figure 3
From: The human pathogenic 91del7 mutation in SLC34A1 has no effect in mineral homeostasis in mice
The 91del7 mutation has no effect on plasma FGF-23 and PTH levels, bone FGF-23 regulatory genes expression and renal protein expression of α-klotho. (A, B) Plasma levels of PTH and intact FGF-23. (C–E) Gene expression of FGF23, Nurr-1 and Galnt3 in femurs. Gene expression was normalized to the housekeeping gene 18S rRNA. (F) Protein expression of α-klotho in renal homogenates. The protein abundance of α-klotho was normalized to β-actin. All parameters were analyzed in samples from 12 weeks old wild type (WT), heterozygous (Het) and homozygous (Hom) 91del7 mice fed standard diet. Data are presented as single values together with mean ± SEM (n = 8 per group). Significance was tested by one-way ANOVA with Bonferroni correction, p ≤ 0.05.
