Figure 4 | Scientific Reports

Figure 4

From: Inosine triphosphate pyrophosphatase from Trypanosoma brucei cleanses cytosolic pools from deaminated nucleotides

Figure 4

Subcellular localization of ITPA in bloodstream T. brucei cells. Immunofluorescence microscopy analysis was performed to establish the subcellular localization of ITPA in parental bloodstream T. brucei cells (BSF) (upper row) and mycTbITPA-overexpressing cells (+ myc-ITPA) (middle and lower row). Nuclear and kinetoplast DNA were stained with DAPI. The mitochondrion was visualized with MitoTracker Red CMXRos and ITPA was detected with anti-ITPA and Alexa Fluor 488 goat anti-rabbit as secondary antibody (upper and middle row) or anti-myc and Alexa Fluor 488 goat anti-mouse as secondary antibody. Images were collected with an Olympus microscope and Cell R IX81 software.

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