Figure 5
Antiproliferative effect of the antiviral ribavirin on trypanosomes is modulated by ITPA and the purines source. (A) Log-phase parasites at 105 cells mL−1 were exposed to increasing concentrations of ribavirin (RBV) for 24 h at 37 °C in HMI-9 medium containing hypoxanthine (1 mM) as the sole purine source. (B) Intracellular detection of RTP by anion exchange chromatography. Top left panel represents the elution standards for ATP and RTP (C) Ribavirin sensitivity assay was performed in modified HMI-9 medium containing guanine (100 μM) as the sole purine source. (D) Ribavirin sensitivity assay was performed in modified HMI-9 medium containing guanine (100 μM) and hypoxanthine (100 μM). Values are the mean (± SD) of at least three independent experiments. Cell lines are represented with the following symbols: BSF (circle), ITPA-KO (square), KO/myc-ITPA in the presence of 1 μg mL−1 of doxycycline (triangle).
