Figure 3
E2 induces CFL1 expression and inhibits p24 expression in HIV-1BaL infected PBMCs. (a) PBMCs were incubated with E2 10,000 pg/ml (vs. untreated control) for 48 h followed by 1000 TCID50/106 cells HIV-1BaL challenge, cultured for 7 days and then incubated on poly l-lysine coated slides for 1 h. PBMCs were then fixed, permeabilized and stained with rabbit anti-human CFL1 Ab and mouse anti-HIV-1-p24 AF488 Ab followed by goat anti-rabbit AF568 Ab and DAPI. Rabbit IgG and goat anti-rabbit AF568 Ab were used as negative controls (NC) for CFL1 staining in uninfected untreated PBMCs. Uninfected untreated PMBCs were used as a NC for p24 staining. Single experiment representative of 3 experiments is shown (original magnification 20X; scale bar is 100 μm). (b) Shown is the normalized fluorescence intensity of CFL1 and p24 staining in relative units (RU) (Mean ± SEM; 3 experiments).
