Figure 3

The chemically defined media that was formulated from the series of Plackett–Burman experiments was modified to include 1 mM Ca²⁺ and 2 mM Mg²⁺ (called CDM2) to support a higher growth rate in Vero cells. CHO-K1, HEK293T and MDCK cells were slowly adapted to CDM2 from serum-containing basal media. All three cell types were able to grow in suspension (triangle) when they were fully adapted to CDM2 and were cultured in shaker flasks. Vero cells remained adherent for all media formulations. Suspension (filled triangle), adherent (open circle).