Figure 3
From: Hydrogen-generating Si-based agent protects against skin flap ischemia–reperfusion injury in rats
HE and TUNEL staining analysis of skin flap after ischemia–reperfusion injury. (a) Representative HE staining images of the superficial and deep layers of the skin flap tissue of each group (scale bar: 100 μm). Inflammatory cell infiltration, hyperemia, and intra-vascular microthrombosis were severe in the I/R group. (b) Tissue damage score evaluation. The score for the I/R + Si group was significantly lower compared to the I/R group. (c) Representative TUNEL staining images of the dermis of the skin flap tissue of each group (scale bar: 20 μm). (d) TUNEL-positive cell evaluation. The percentage of TUNEL-positive cells in the I/R + Si group was significantly lower compared to the I/R group (*p < 0.05, **p < 0.01, ***p < 0.001; Tukey’s HSD multiple comparisons test, bars represent standard errors of mean).
