Figure 3
(A): Genes related positive regulation of cell cycle (red), and negative regulation of cell cycle (blue) were plotted on the volcano plot. (B): Cell proliferation assay. We passaged the cells three times every 2 days (days 2, 4, and 6) and counted the number of the cells at each passage. (control: n = 5, CAM: n = 4) *p < 0.05, **p < 0.01, Error bars indicate ± SE. Student’s t test was used for statistical analysis. (C): MTS assay of UCMSCs statistically revealed the significant difference between the control (n = 3) and the CAM group (n = 3). *p < 0.05, Error bars indicate ± SE. Student’s t test was used for statistical analysis. (D): Representative data of the cell cycle distribution of the UCMSCs from the control and the CAM group. Compared with the control group, the cells in G2/S phase were decreased in the CAM group. (E): A comparison of the UCMSCs population at each phase of the cell cycle revealed the significant difference between the control (n = 4) and the CAM group (n = 4), ***p < 0.001, Error bars indicate ± SE. Student’s t test was used for statistical analysis.
