Figure 6 | Scientific Reports

Figure 6

From: Alterations of mucosa-attached microbiome and epithelial cell numbers in the cystic fibrosis small intestine with implications for intestinal disease

Figure 6

Expression of stem, goblet and Paneth cells in human ileal tissues from one patient with CF and seven non-CF controls. Quantification of cell numbers and representative images of (A) stem cells (leucine-rich repeat-containing G-protein coupled receptor 5, LRG5) positive cells stained red; (B) goblet cells (mucin-2, MUC2) positive cells stained green and (C) Paneth cells (defensin 5, DEFA5) positive cells, stained red. Nuclei are stained blue with 4ʹ, 6-diamidino-2-phenylindole (DAPI). Box and whisker plots represent total number of cells counted expressing LRG5, MUC2 and DEFA5 as median ± 95% CI. The insets enclosed in white boxes show higher magnification of the areas enclosed by the dashed lines. Images are 400 × (A), and 100 × (B) & 200 × (C) magnified.

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