Figure 1
Assessment of biochemical parameters and histological examination following porcine renal ischemia/reperfusion injury. (A) Experimental design of renal unilateral IRI following contralateral nephrectomy. Ischemia was induced for 30 min. Data were collected before injury, 5 min and 7 days following renal clamping. (B) Measurement of blood urea nitrogen (BUN) and serum creatinine (SCr) levels in males (blue) and females (red). The “y” axis represents blood urea nitrogen and serum creatinine concentration, respectively, and the “x” axis represents the time points. Average values ± SEM are plotted in the graph (N = 5). (C) Representative images of different levels of tubular injury and interstitial infiltration in pig kidney. Arrows indicate specifically damaged cells. Magnification = 20X, scale bar = 100 µm. (D) Quantification of tubular injury (upper panel) and interstitial infiltration (lower panel) scored by an expert pathologist was classified by group and sex (males in blue, females in red). The y-axis represents the % of animals showing each level of injury or infiltration, respectively. BUN blood urea nitrogen, PR pre-ischemia, PS post-ischemia, WL one week later. *p < 0.05.
