Figure 3
Validation of porcine renal IRI microarray assays by qRT-PCR experiments followed by evaluation of mRNA levels of selected targets in human ischemic kidney biopsies. (A) Expression values of five selected targets from microarray assays displaying time and sex differences. (B) Relative mRNA levels of FABP5, IFIT3, RSAD2, CXCL10, CD274 were measured and compared by qRT-PCR. For the time comparison, the different time points (PR, PS, WL) were compared with each other for each sex (blue male, red female). For the sex comparison, a selected time point in male was compared to the equivalent time point in female. Blue and red lines represent a time comparison in male or female, respectively. Black lines represent sex comparison at equivalent time points. (C) RSAD2, CXCL10, CD274, FABP5 and IFIT3 expression levels were evaluated by qPCR in post-surgery (PS) conditions from samples of 36–80 and 53–83 years old men and women, respectively (N > 7). *p value ≤ 0.05; **p value ≤ 0.01; ***p value ≤ 0.001; ****p value ≤ 0.0001, ns: not significant. F female, M male, PR pre-ischemia, PS post-ischemia, WL one week later.
