Table 1 Treatments to induce maturation and spawning in flathead grey mullet (Mugil cephalus) females and the results obtained.

Treatment priming injection

30 µg kg⁻¹ rLh

30 µg kg⁻¹ rLh

40 mg kg⁻¹ P₄

Treatment resolving injection

40 mg kg⁻¹ P₄

30 µg kg⁻¹ rLh

40 mg kg⁻¹ P₄

Oocyte diameter (µm)

606 ± 10^a

602 ± 6^a

600 ± 7^a

No. of injected females

9

6

6

No. of spawned females

8

6

1

Latency period (h)

16:36 ± 1:56^a

16:51 ± 2:22^a

24:30:00

Ovulation success (%)

100

100

50

Spawning success (%)

89

100

17

Total eggs per female

1,760,100 ± 821,102^a

1,631,889 ± 954,138^a

1,280,833 ± 966,42^a

Fecundity (egg kg⁻¹ bw)

1,194,637 ± 635,747^a

1,205,1387 ± 487,002^a

694,529 ± 526,457^a

Initial fertilization (%)

49 ± 23^a (8 min–80 max)

60 ± 16^a (39 min–84 max)

0

Embryo survival (%)

56 ± 22^a (15 min–80 max)

73 ± 21^a (42 min–90 max)

0

Hatching (%)

57 ± 21^a (25 min–88 max)

61 ± 30^a (23 min–90 max)

–

Hatching 96-well plate (%)

95 ± 4^a

97 ± 1^a

–