Figure 2 | Scientific Reports

Figure 2

From: Generation of 3D retinal tissue from human pluripotent stem cells using a directed small molecule-based serum-free microwell platform

Figure 2

Small molecule-based 2D/3D differentiation of hPSC into retinal organoids under xeno-free condition. (A) Schematic of the 2D/3D xeno-free small molecule protocol (B) Bright-field microscopy of 3D optic vesicle-like structure at day 30 and manually picked organoid at day 60 of differentiation (scale bars 100 µm) (C) RT PCR analysis of relative expression of retinal marker genes (mean ± SD; n = 3 independently cultured sets of 10 OVs from N = 1 ESC line, MShef10). (D) Immunohistological analysis of retinal organoids at week 17 of differentiation (generated from MShef10 ESC line; scale bars 100 µm). (E) Immunohistological analysis of CRX, RCVRN, OPN1MW/LW, and RHO in retinal organoids at week 33 of differentiation (generated from MShef10 ESC line). (F) Immunohistological analysis of CRX, RCVRN, OPN1MW/LW, ARR3, RHO, GNAT, PRPH2, ARL13B in retinal organoids (from MShef10 ESC line) at week 36 of differentiation.

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