Figure 5 | Scientific Reports

Figure 5

From: Automated quantitative high-throughput multiplex immunofluorescence pipeline to evaluate OXPHOS defects in formalin-fixed human prostate tissue

Figure 5

Intra-patient heterogeneity in mitochondrial marker expression. Characterisation of randomly selected regions of interest (ROIs) from patient P14. (A) ROIs annotated on a pan-cytokeratin labelled immunofluorescence image (4× objective, scale bar represents 2 mm). The purple region highlights tumour-rich tissue. Each blue/yellow box represents a ROI with adjacent numbers labelled for ROIs selected in panel (C). (B) Single-cell level quantification of mitochondrial marker abundance across all ROIs within P14 demonstrates widespread spatial variation amongst tumour ROIs, sorted by NDUFB8 Z-score and histopathological subgroup (n = 39,609 cells from 42 ROIs). (C) Representative ROIs from each histopathological subgroup. Each panel includes a top row of fluorescence-labelled images. The lower row contains corresponding haematoxylin & eosin stained images and pseudo-images representing single-cell level z-scores in epithelial cells, with ROI mean Z-score denoted above. High magnification insets (50 μm × 50 μm) are included. Scale bar represents 100 μm.

Back to article page