Table 1 Antibodies used in automated and manual multiplex immunofluorescence.

1°

α-NDUFB8

Complex I subunit

Mouse IgG1

ab10242

Abcam

1:100^a,m

1°

α-MTCO1

Complex IV subunit

Mouse IgG2a

ab14705

Abcam

1:500^a

1:100^m

1°

α-TOMM20

Mitochondrial mass marker

Rabbit IgG

ab186734

Abcam

1:500^a

1:100^m

1°

α-Pan-cytokeratin

Epithelial cell marker

Mouse IgG1

C-2931

Sigma Aldrich

1:10,000^a

1:400^m

2°

Biotin-conjugate

Mouse IgG1

Goat

115-065-205

Jackson IR lab

1:200^m

2°

AlexaFluor 488

Mouse IgG2a

Goat

A21131

Life Technologies

1:200^m

2°

AlexaFluor 546

Rabbit IgG

Goat

A175732

Life Technologies

1:200^m

3°

AlexaFluor 647

Streptavidin

Goat

S1125

Life Technologies

1:200^m

2°

AlexaFluor 750

Mouse IgG H + L

Goat

A175732

Life Technologies

1:200^m

2°

Hoechst 33342

DNA

Synthetic

H3570

Life Technologies

1:1400^m

2°

Spectral DAPI

HRP

Synthetic

FP1490A

Akoya Biosystems, Marlborough, USA

1 drop in 500 ul^a

2°

Opal 520

HRP

Synthetic

FP1487A

1:100^a

2°

Opal 570

HRP

Synthetic

FP1488A

1:500^a

2°

Opal 620

HRP

Synthetic

FP1495A

1:150^a

2°

Opal 650

HRP

Synthetic

FP1496A

1:150^a

2°

Opal 690

HRP

Synthetic

FP1497A

1:150^a