Figure 1
The antibody induced by sEH vaccine inhibited hydrolysis of EETs. (a) The peptide sequence of sEH vaccine. (b) The protocol of vaccination and evaluation. The sEH vaccine was administered to 5-wk-old rats 4 times at 2-wk intervals. (c) Anti-sEH antibody titer was quantified by ELISA. A high half-maximum value was obtained (> 1000) in all four rats after the second vaccination. n = 4. *P < 0.05. (d) Blood from rats was collected at 14 wk of age and preserved as plasma. Having the antibody in the plasma prevented 14,15-EET-d11, 11,12-EET-d11 and 8,9-EET-d11 from being hydrolyzed into 14,15-DHET-d11, 11,12-DHET-d11 and 8,9-DHET-d11 and the ratio of 14,15-EET-d11 to 14,15-DHET-d11, 11,12-EET-d11 to 11,12-DHET-d11 and 8,9-EET-d11 to 8,9-DHET-d11 were increased in the vaccinated group. n = 8 in the control group and n = 9 in the vaccinated group. **P < 0.05.
