Figure 4
sEH vaccine treatment induced neovascularization in ischemic border area. (a) Immunofluorescent staining of (a) lectin, (b) CD31, and (c) α-smooth muscle actin. The nuclei were visualized using Hoechst 33342. Sections of hearts from the saline group (n = 5–7) and the vaccinated group (n = 5) at day 7 were evaluated. Scale bar = 100 μm. The border areas between infarcted and non-infarcted sites were randomly measured at five locations. Cells labeled with CD31, a marker of endothelial cells, were counted (cells/mm3). *P < 0.05 Lectin was also used to assess vascular density (%). *P < 0.05.
