Figure 2
Slice electrophysiology profile of DCZ and CNO in CeA neurons expressing hM4Di DREADDs. (A) Atlas schematic depicting viral expression of hM4Di.mCherry in CeA neurons targeted for slice electrophysiology recordings. (B) Left: Concentration–response curve depicting percent inhibition of firing rate in CeA neurons expressing hSyn.hM4Di.mCherry during bath application of varying concentrations of either DCZ or CNO. Right: Representative traces of action potential firing in hM4Di-expressing neurons at baseline (top) or during bath application of 1000 nM DCZ (bottom). Average changes in action potential firing during 1000 nM application of DCZ (C) or CNO (D) in individual animals. (E) Average change in membrane potential from bath application of various drug concentrations *p < 0.05, #p = 0.0549; n = 4–5 rats/group.
