Figure 7

(a) Results of principal component analysis of gene expression profiles in 4TO7 cells treated with vehicle (ctl), AM-193, DK-406, SAHA (S), 1,25D (VD) or SAHA + 1,25D (SVD) for 6 or 24 h. (b) Venn diagrams showing overlaps in expression profiles of upregulated genes in 4TO7 cells treated with AM-193, 1,25D, SAHA or 1,25D + SAHA for 6 or 24 h. (c) Top enriched diseases and biological functions following 6 and 24 h treatment with 1,25D, 1,25D + SAHA, DK-406 or AM-193. Diseases and functions with a predicted activation state (positive z-score) are labeled in orange while those with a predicted inhibition state (negative z-score) are labeled in blue. All diseases and functions have a log p-value cut-off of 1.3. Enclosed in red rectangles are diseases and biological functions that are of interest. (d) Selected diseases and biological functions that are predicted to be activated (positive z-score) and inhibited (negative z-score) subsequent to 24 h treatment with AM-193. Each function is displayed as nodes (genes) and edges (biological relationship between nodes). The colour intensity of each node signifies log fold change expression; red and green represent upregulated and downregulated genes, respectively. Edges connecting the genes to the respective functions indicate the predicted relationships. The colour of the central function and edges indicate activation (orange) or inhibition (blue) of the different functions. Inconsistent findings and effects that are not predicted are not illustrated for clarity.