Figure 2
From: Functional identification of microRNA-centered complexes in C. elegans
miRNA pulldowns identify components of translation machinery and mRNA processing factors, which may form a functional network. (a–d) Terms identified through GO analysis for biological processes (top four), cellular components (top four) and molecular function categories (top three) among let-7 PDs in (a) wild type, (b) mir-48 mir-241(nDf51); mir-84(n4037) backgrounds, (c) miR-58 PD and (d) ALG-1 IP19. Total number of proteins classified under each term shown adjacent to respective bars. (e–h) RNA binding domains identified in factors captured in let-7 PDs from (e) wild type, and (f) mir-48 mir-241(nDf51); mir-84(n4037)) backgrounds, (g) miR-58 PD and (h) ALG-1 IP. The FDR adjusted p-values for enrichment of proteins harboring individual domains are shown within the respective bars. (i–l) The reproducibly enriched proteins form functional network. Network analysis was performed using STRING28 on top 40 enriched interactors in (i) let-7 PD (wild type), (j) let-7 PD (mir-48 mir-241(nDf51); mir-84(n4037)) (k) miR-58 PD and (l) ALG-1 IP. Ribosomal proteins were excluded from this analysis. Thickness of edges represents degree of confidence of functional linkages. The number of edges is significantly higher than expected, with a p-value < 1.0e−16.
